human placental pericytes  (PromoCell)

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: TGF β -1 signalling via canonical receptor ALK5 in placental pericytes. ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Expressing, Clinical Proteomics, Membrane, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: ELISA analysis of angiogenic factors secreted from placental pericytes. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in; ( a ) VEGF secretion (Kruskal–Wallis analysis); ( b ) MMP-2 secretion (one-way ANOVA). ( c ) ANG-1 secretion (one-way ANOVA). ELISA results normalized to cell number. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Collagen IV, fibronectin and laminin staining in placental pericytes. Representative ICC/IF images (VEH-treated placental pericytes); ( a ) Collagen IV (red), nuclear stain (blue); data expressed as area and mean fluorescent intensity (MFI); one-way ANOVA, normalized to cell number; ( b ) Fibronectin (red), nuclear stain (blue), data expressed as area and MFI; one-way ANOVA, normalized to cell number; ( c ) Laminin (red), nuclear stain (blue); data expressed as area; Kruskal–Wallis analysis and MFI; one-way ANOVA, normalized to cell number. Scale Bars = 150 μm. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Staining

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Placental pericyte secretion of inflammatory factors. Analysis of inflammatory secretion (ELISA). Treatment groups: VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( a ) IL-6 secretion; one-way ANOVA; ( b ) MCP-1 secretion; one-way ANOVA; ( c ) sVCAM-1 secretion; one-way ANOVA; ( d ) CX3CL1 secretion; one-way ANOVA. ( e ) IL-8 secretion; Kruskal–Wallis analysis. Data (n = 3); stars indicate statistical significance between treatment groups ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Alternate TGF β -1 signalling pathway in placental pericytes: Type I receptor ACVRL1 (ALK1, 94 bp) expression in placental pericytes, as assessed by RT-PCR; original image shown.

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Phagocytosis capacity of placental pericytes. Treatment groups: VEH control and TGF β -1 (10 ng/mL) with and without FluoSphere incubation; ( a ) Representative flow cytometry histograms to identify the percentage of the population with and without phagocytosed FluoSpheres based on fluorescence; ( b ) Analysis of phagocytosis (Mann–Whitney). Data (n = 3) expressed as mean ± SEM. Stars indicate statistical significance, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; c Fluorescent and phase contrast images of pericyte phagocytosis, nuclei (blue), FluoSphere (green). Yellow arrows identify pericytes with phagocytosed beads, and red arrows identify beads that have not been phagocytosed. Scale bar = 150 μm.

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Control, Incubation, Flow Cytometry, Fluorescence, MANN-WHITNEY

Journal: Lab on a chip

Article Title: Late-stage placental barrier model for transport studies of prescription drugs during pregnancy.

doi: 10.1039/d5lc00075k

Figure Lengend Snippet: Fig. 3 Effects of pericytes on vascular and total permeability of placental barrier model on days 16 and 18 of culture. A. Brightfield images of vascular channel for the following conditions: no cell control, ST only, ST + HUVECs, and two tricultures with ST + HUVECs + hPC-PLs at high and low density. Fluorescent images of 4 kDa (green) and 65 kDa (red) dextran permeation through the endothelial channel after 1 hour of perfusion. B. Vascular barrier permeability to 4 kDa dextran. (N = 3–7, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001) C. Vascular barrier permeability to 65 kDa dextran. (N = 3–7, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001) D. Fluorescent images of 65 kDa dextran (red) permeating from the central maternal compartment, through the ST and HUVEC barriers, to the adjacent fetal compartments after 24 hours. E. Total barrier permeability to 4 kDa dextran from the maternal to the fetal compartments. (N = 6–7, one-way ANOVA, **p < 0.01, ***p < 0.001) F. Total barrier permeability to 65 kDa dextran from the maternal to the fetal compartments. (N = 6–7, one-way ANOVA, ***p < 0.001).

Article Snippet: Primary human placental pericytes (hPC-PL) were obtained from PromoCell (C-12980), cultured in pericyte growth medium 2 (PGM2, Millipore Sigma, C-28041) and used until passage 5.

Techniques: Permeability, Control

Journal: Placenta

Article Title: Characterizing placental pericytes: Hypoxia and proangiogenic signalling.

doi: 10.1016/j.placenta.2024.07.314

Figure Lengend Snippet: Fig. 1. Human placental pericyte cell count and viability in 20 % O2 and 1 % O2 (n = 3 per treatment group). Placental pericyte cell count (A) and viability (B) were unchanged in 1 % O2. Statistical analysis compared cell count/viability in 20 % O2 and 1 % O2 using unpaired t-tests. Data expressed as mean ± SEM. Stars indicate statistical significance between treatment groups, *p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001.

Article Snippet: Primary human placental pericytes (PromoCell) were seeded at 3.0 × 103 cells/cm2 in Basal Medium (PromoCell) supplemented with supplier-provided SupplementMix and used up to passage 6 (recommended by supplier).

Techniques: Cell Counting

Journal: Placenta

Article Title: Characterizing placental pericytes: Hypoxia and proangiogenic signalling.

doi: 10.1016/j.placenta.2024.07.314

Figure Lengend Snippet: Fig. 2. Angiogenic secretion of human placental pericytes in 20 % O2 and 1 % O2 (n = 3 per treatment group). ELISAs performed on cell culture supernatant. (A) Placental pericyte secretion of ANG1 was decreased in 1 % O2. (B) Placental pericyte secretion of VEGF was increased in 1 % O2. (C) Placental pericyte secretion of MMP2 was unchanged in 1 % O2. Statistical analysis compared secreted angiogenic factor concentration in 20 % O2 and 1 % O2 using unpaired t-tests. Data expressed as mean ± SEM. Stars indicate statistical significance between treatment groups, *p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001.

Article Snippet: Primary human placental pericytes (PromoCell) were seeded at 3.0 × 103 cells/cm2 in Basal Medium (PromoCell) supplemented with supplier-provided SupplementMix and used up to passage 6 (recommended by supplier).

Techniques: Cell Culture, Concentration Assay

Journal: Placenta

Article Title: Characterizing placental pericytes: Hypoxia and proangiogenic signalling.

doi: 10.1016/j.placenta.2024.07.314

Figure Lengend Snippet: Fig. 5. PlGF and sFLT1 secretion of human placental pericytes, HUVECs, BeWos (cytotrophoblasts), and syncytialized BeWos in 20 % O2 and 1 % O2 (n = 3 per treatment group). ELISAs performed on cell culture supernatant. (A) HUVEC and syncytialized BeWo secretion of PlGF were increased in 1 % O2. (B) HUVEC, BeWo (cytotrophoblast), and syncytialized BeWo secretion of sFLT1 were increased in 1 % O2, while placental pericyte secretion of sFLT1 was decreased in 1 % O2. Statistical analysis compared secreted angiogenic factor concentration in 20 % O2 and 1 % O2 using unpaired t-tests for each cell type. Data expressed as mean ± SEM. Stars indicate statistical significance between treatment groups, *p ≤0.05, **p ≤0.01, ***p ≤0.001, ****p ≤0.0001.

Article Snippet: Primary human placental pericytes (PromoCell) were seeded at 3.0 × 103 cells/cm2 in Basal Medium (PromoCell) supplemented with supplier-provided SupplementMix and used up to passage 6 (recommended by supplier).

Techniques: Cell Culture, Concentration Assay

Journal: Placenta

Article Title: Characterizing placental pericytes: Hypoxia and proangiogenic signalling.

doi: 10.1016/j.placenta.2024.07.314

Figure Lengend Snippet: Fig. 6. Double IF on HUVEC- and human placental pericyte-derived vascular sprout. 200× magnification of HUVEC and human placental pericyte-coated bead and vascular sprout stained for nuclei (A, NucBlue, blue), endothelial cells (B, VE cadherin, red) and pericyte cells (C, αSMA, green). Human placental pericytes colocalize with HUVECS on the endothelial sprouts in vitro (D, merge). Scale bar = 150 μm.

Article Snippet: Primary human placental pericytes (PromoCell) were seeded at 3.0 × 103 cells/cm2 in Basal Medium (PromoCell) supplemented with supplier-provided SupplementMix and used up to passage 6 (recommended by supplier).

Techniques: Derivative Assay, Staining, In Vitro

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Mimicking angiogenic microenvironment of alveolar soft-part sarcoma in a microfluidic coculture vasculature chip.

doi: 10.1073/pnas.2312472121

Figure Lengend Snippet: Fig. 3. Angiogenic microenvironment of ASPS in a coculture (5:1) vasculature chip. (A) Illustration of the coculture (5:1) vasculature device before and after immunohistochemical treatment on day 11. (B) Illustration of ASPS-wrapped PC and EC in the ASPS tumor microenvironment. (C) Orthogonal views at the bottom plane and (D) their magnification of confocal images of an AS17 spheroid with coculture ECs:PCs (5:1) on day 11 in the microfluidic device (white dashed box). White arrowheads indicate the attachment of cells to pericytes surrounding the tumor vessel. (E) Orthogonal views at the middle plane and (F) their magnification of confocal images in the same device. White arrowheads indicate the localization of ASPS cells that surround the pericytes. (G) Orthogonal views at the top plane and their magnification of (H) y-z, and (I) x-z planes in the same device. White arrowheads indicate cell–cell crosstalk of ASPS surrounding the pericytes that wrap the tumor vessel. (J) Z-projection image of the device. The 3D vasculature with pericytes penetrates the spheroid core. In (C–J), anti-Flag detects Flag- tagged ASPSCR1-TFE3 in ASPS cells (blue), anti-CD31 detects CD31-mediated endothelial cell-to-cell interaction (green), and anti-alpha-smooth muscle actin detects microfilament bundles of pericytes (red).

Article Snippet: Human placental microvascular pericytes (HPMPCs, Angio Proteomie) were cultured in pericyte growth medium (PGM, super rich formulation, Angio- Proteomie) and the sixth passage was used for experiments.

Techniques: Immunohistochemical staining

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Mimicking angiogenic microenvironment of alveolar soft-part sarcoma in a microfluidic coculture vasculature chip.

doi: 10.1073/pnas.2312472121

Figure Lengend Snippet: Fig. 6. Signaling proteins delivered with the aid of intracellular trafficking proteins in the coculture (20:1) vasculature chip. Representative fluorescence images of an in vitro AS17 spheroid with accumulation of Pdgfb in different tumor microenvironments; (A) no vasculature, (B) monoculture vasculature (EC alone), and (C) coculture ECs:PCs (20:1). The ASPS cells were determined by immunofluorescence staining with DAPI detecting nuclei (yellow), anti-PDGF B antibody detecting Pdgfb (red), and Anti-flag® M2 antibody detecting Flag-tagged ASPSCR-TFE3 (blue). Representative fluorescence images of an in vitro AS17 spheroid with the accumulation of Gpnmb in different tumor microenvironments; (D) no vasculature, (E) monoculture vasculature (EC alone), and (F) coculture ECs:PCs (20:1). The ASPS cells were determined by immunofluorescence staining with DAPI detecting nuclei (yellow), mouse oseteoactivin/GPNMB antibody detecting Gpnmb (red), and Anti-flag® M2 antibody detecting Flag-tagged ASPSCR1-TFE3 (blue). (G) H&E staining and immunohistochemical analysis of an in vivo ASPS tumor. H&E showed ASPS cells expressing ASPSCR1-TFE3 surrounded by pericytes (arrowhead). Immunostaining images showed the Flag-tagged ASPSCR1-TFE3 marker for ASPS cells and Gpnmb, and Pdgfb markers for accumulation of Gpnmb and Pdgfb proteins in ASPS cells.

Article Snippet: Human placental microvascular pericytes (HPMPCs, Angio Proteomie) were cultured in pericyte growth medium (PGM, super rich formulation, Angio- Proteomie) and the sixth passage was used for experiments.

Techniques: Fluorescence, In Vitro, Immunofluorescence, Staining, Immunohistochemical staining, In Vivo, Expressing, Immunostaining, Marker